I downloaded and installed the MS AnitSpyware beta a couple of days ago. I ran a scan and removed everything the software identified as a problem. Now, when I boot up, I get a message box titled "16 Bit Subsystem" that says AUTOEXEC.NT is not suitable for running DOS or Windows Applications.
Fix submitted by Jim Byrd
Try this fix, courtesy of MVP Kelly Theiriot:
"This installaton program will reinstall the missing or corrupt Windows XP system files command.com, autoexec.nt and config.nt. The absence or corruption of one or more of these files causes a "16 Bit Subsystem" error.
XP_Fix.EXE by TRF Systems, Inc.
More info here:
Error Message: MS-DOS or 16-bit Windows-based program
Then, courtesy of flrman1, here:
"First in safe mode click on My Computer then click Tools > Folder Options. In Folder options click on the View tab. Under Files and Folders tick "Show hidden files and folders" then uncheck "Hide file extensions for known file types" and uncheck "Hide protected operating system files (recommended)". Now click "Like current folder" then "Apply" and "OK"
Now find and delete these files:
C:\WINNT\system\windupdate.exe (added by JB: note the spelling – the added ‘d’)
Delete this folder:
Also in safe mode navigate to the C:\Windows\Temp folder. Open the Temp folder and go to Edit > Select All then Edit > Delete to delete the entire contents of the Temp folder.
Go to Start > Run and type %temp% in the Run box. The Temp folder will open.
Click Edit > Select All then Edit > Delete to delete the entire contents of
the Temp folder.
Finally go to Control Panel > Internet Options. On the General tab under "Temporary Internet Files" Click "Delete Files". Put a check by "Delete Offline Content" and click OK. Click on the Programs tab then click the
"Reset Web Settings" button. Click Apply then OK.
Empty the Recycle Bin"
Run the fix file again after you’re done w/the above.